Shoot Induction of Pandanus Tectorius through Shoot Culture in WPM Medium Supplemented With BAP And NAA

Abstract

The aim of this research is to determine the best shoot induction medium for Pandanus tectorius, a coastal plant valued for its ecological and economic benefits. Coastal degradation and land conversion have reduced its population, necessitating propagation efforts. In vitro culture was explored as a solution to address the scarcity of Pandanus tectorius. Woody Plant Medium (WPM) was prepared by combining 100 mL stock solution, 30 g sugar, and distilled water to 1 L, supplemented with 1 g activated charcoal, 0.1 g ascorbic acid, and plant growth regulators at varying concentrations. The pH was adjusted to 5.6–5.8, and agar was added before sterilization at 121 °C for 20 minutes. Explants consisting of 1 cm-long shoots were sterilized and cultured in bottles, with observations conducted over 8 weeks. Results showed that the best shoot induction was achieved using WPM supplemented with 5x10⁻⁶ M BAP and 5x10⁻⁶ M NAA, producing 1 to 3 shoots per explant. Data analysis using a factorial completely randomized design confirmed significant differences among treatments. This study demonstrates the effectiveness of in vitro propagation in restoring Pandanus tectorius populations and supports sustainable coastal management.